《植物生理学报》 2015, 51(4): 528-536

玉米BSKs基因家族生物信息学分析

胡雪微^1,2, 杨克军^1,2,*, 李佐同^1,2, 张海燕^1,2, 刘鑫¹, 赵莹^1,2, 聂江山¹, 韩雨^1,2, 赵长江^1,2,*

¹黑龙江八一农垦大学农学院, 黑龙江大庆163319; ²黑龙江省普通高校寒地作物种质改良与栽培重点实验室, 黑龙江大庆163319

通信作者：杨克军；E-mail: byndykj@163.com, zhaocj15@126.com；Tel: 0459-6819170, 0459-6819181

摘　要：

油菜素内酯信号激酶(BSKs)是一类参与油菜素内酯(BR)信号转导的植物特异受体类胞质激酶。本研究采用生物信息学的方法在玉米基因组水平鉴定玉米BSKs基因家族, 对基因结构、启动子元件、蛋白质结构和性质、基因表达模式及候选互作蛋白等进行分析。鉴定的14个玉米BSKs基因分别定位在玉米的1、2、4、5和9号染色体上, 1号染色体上多达6个; 编码蛋白包含典型的PKc和TPR结构域, N端由延长链和螺旋结构组成, 定位于叶绿体, 多为亲水性、酸性蛋白, 可能与热激蛋白、含CS结构域蛋白及受体BRI1互作。玉米BSKs基因在从种子萌发到成熟的整个生长发育期, 以及不同组织和器官呈现不同的表达模式; 基因启动子上含有大量激素和非生物逆境诱导的顺式作用元件, 其中激素SA及NaCl诱导相关元件数量最多, 表明玉米BSKs基因可能在不同生育期受多种激素调控, 并参与玉米对多种逆境的应答调控。该研究有助于解析单子叶C4作物玉米BSKs基因在生长发育及逆境胁迫响应中的功能。

关键词：玉米; BSK; BSKs基因家族; 生物信息学

收稿：2014-12-11   修定：2015-03-23

资助：国家自然科学基金(31101429)、教育部博士学科点博导类专项科研基金(20122305110001)、黑龙江省自然科学基金项目(C201324)、黑龙江省教育厅科学技术研究项目(12511360)、黑龙江八一农垦大学研究生创新项目(YJSCX2014-Y02)和黑龙江省大学生创新创业训练计划项目(2012)。

Genome-Wide Analysis of the BSKs Gene Family in Maize (Zea mays)

HU Xue-Wei^1,2, YANG Ke-Jun^1,2,*, LI Zuo-Tong^1,2, ZHANG Hai-Yan^1,2, LIU Xin¹, ZHAO Ying^1,2, NIE Jiang-Shan¹, HAN Yu^1,2, ZHAO Chang-Jiang^1,2,*

¹College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; ²Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilongjiang Province Education Department, Daqing, Heilongjiang 163319, China

Corresponding author: YANG Ke-Jun; E-mail: byndykj@163.com, zhaocj15@126.com; Tel: 0459-6819170, 0459-6819181

Abstract:

Brassinosteroid signaling kinases (BSKs) are novel plant specific receptor-like cytoplasmic kinases involved in the brassinosteroid (BR) signal transduction pathway. In this study, a family of maize (Zea mays) BSKs genes was identified from maize genome using bioinformatic methods, and their structures, promoter elements, protein structure and properties, gene expression pattern and candidate interacting proteins were analyzed. The results revealed that 14 maize BSKs genes located on chromosome 1, 2, 4, 5 and 9, respectively, with up to 6 of them on chromosome 1. The encoded proteins had typical PKc and TPR motifs and a complicated N-terminal made up of extending chains and helixes; they localized in chloroplast and most of them were hydrophilic and acidic, interacting probably with heat shock proteins, proteins with CS domain and BRI1. Differential expression profiles of maize BSKs genes were observed on developmental stages from germination to maturity, and in different tissues. A great deal of cis-elements related to hormones and abiotic stresses were uncovered in the promoter regions, and most of them were found to be salicylic acid (SA) and salt (NaCl) induced, which suggested that the maize BSKs genes were modulated by multiple hormones under different developmental stages, and might play a role in response to many kinds of abiotic stresses. This study will help to decipher the functions of BSKs genes from monocots C4 crop maize on growth, development and responses to abiotic stresses.

Key words: maize; BSK; BSKs gene family; bioinformatic analysis